Composition of lectin receptors in serous and mucinous ovarian cystadenomas

Authors

DOI:

https://doi.org/10.18370/2309-4117.2016.29.16-18

Keywords:

serous cystadenoma, mucinous cystadenoma, lectins, glycoconjugates, proliferation

Abstract

The most common group of benign cystic ovarian tumors in women of all ages are epithelial tumors (cystadenomas) that make up about a third of ovarian tumors and half of benign lesions.

The growth of tumor proliferative activity accompanied by increased number of lectins receptors in cells. Lectins are non-immune origin proteins that have a common property of binding carbohydrates and carbohydrate determinants of biopolymers strictly defined structure and intact their covalent structure. It was established that oncogenesis changes of the structure of glycoconjugates membrane as cancer and immune cells, and mark faster than glycoconjugates variability in tumor cells. This is the basis for the use of labeled lectins to study changes in cell membrane and secretory structures of glycoconjugates during tumor growth.

The aim of the study was to determine the composition of the receptors in serous and mucinous cystadenomas.

Operating material was studied from 60 women operated on for ovarian serous cystadenomas, and from 60 patients – on for ovarian mucinous cystadenomas. To identify the set of glycoconjugates it were used peroxidaselabeled lectins with different carbohydrate specificity.

Characteristic of serous and mucinous cystadenomas was the accumulation of lectins receptors in their cells of wheat germs (WGA), groundnut seed (PNA) and soybean seeds (SBA). Papillary serous cystadenomas differed moderate accumulation in the epithelium papillae throughout the cytoplasm lectins receptors of SBA, mistletoe (VAL) and WGA. The most intense and uniform expression in mucinous cystadenomas was one of lectin receptors of bark elderberry (SNA) and SBA.

The authors conclude that changes in the molecular-spatial structure of glycoconjugates of the plasmolemma cells surfaces and increase of the mobility of membrane lectins receptors in serous and mucinous cystadenomas cause lack of contact inhibition of proliferation. In their opinion, further study of lectins can contribute to the elucidation of the pathogenesis of ovarian formations, their differential diagnosis, the development of tumor markers and drugs lectins.

Author Biographies

O. M. Nosenko, Odesa National Medical University

MD, professor at the Obstetrics and Gynecology Department № 1

V. G. Dubinina, Odesa National Medical University

MD, professor, head of the Oncology Department with a Course of Radiation Diagnostics, Therapy and Radiation Medicine

M. A. Lysenko, Odesa National Medical University

Postgraduate student of the Obstetrics and Gynecology Department № 1

References

  1. Nosenko, O.M. Benign cystic formation of ovary: epidemiology, pathogenesis, diagnosis and recovery of reproductive health. The thesis for submitting an academic degree of doctor of medical sciences: 14.01.01. Kyiv (2008): 42 p.
  2. Yashchenko, A.M. Lectins as histochemical markers in health and disease. The thesis for submitting an academic degree of doctor of medical sciences: 14.03.09. Kyiv (2004): 35 p.
  3. Varki, A., Cummings, R.D., Esko, J.D., Freeze, H.H., et al. Essentials of Glycobiology. 3rd edition. Cold Spring Harbor (NY). Cold Spring Harbor Laboratory Press (2015): 800 p.
  4. Yakovtsova, I.I. “Distribution of lectin receptors in the epithelium of serous ovarian tumors.” Archive for Clinical and Experimental Medicine 4.1 (1995): 22–24.
  5. Thiel, S., Kolev, M., Degn, S., Steffensen, R., et al. “Polymorphisms in mannan-binding lectin (MBL)-associated serine protease 2 affect stability, binding to MBL, and enzymatic activity.” J Immunol 182.5 (2009): 2939–47. DOI: 10.4049/jimmunol.0802053
  6. Allam, H., Aoki, K., Benigno, B.B., McDonald, J.F., et al. “Glycomic analysis of membrane glycoproteins with bisecting glycosylation from ovarian cancer tissues reveals novel structures and functions.” J Proteome Res 14.1 (2015): 434–46. DOI: 10.1021/pr501174p
  7. Takahashi, M., Kuroki, Y., Ohtsubo, K., Taniguchi, N. “Core fucose and bisecting GlcNAc, the direct modifiers of the N-glycan core: their functions and target proteins.” Carbohydr Res 344.12 (2009):1387–90. DOI: 10.1016/j.carres.2009.04.031
  8. Gnedkova, I.A., Lisyanyi, N.I., Stanetskaya, D.N., et al. “Binding of lectins and tumorigenic properties of glioma cells C6.” Oncology 17.1 (2015): 4–11.
  9. Anugraham, M., Jacob, F., Nixdorf, S., et al. “Specific glycosylation of membrane proteins in epithelial ovarian cancer cell lines: glycan structures reflect gene expression and DNA methylation status.” Mol Cell Proteomics 13.9 (2014):2213–32. DOI: 10.1074/mcp.M113.037085
  10. Rodriguez-Paredes, M., Esteller, M. “Cancer epigenetics reaches mainstream oncology.” Nat Med 17 (2011): 330–9.
  11. Antoniuk, V.O. Lectins and their sources of raw materials. Lviv. Quart (2005): 554 p.

Downloads

Published

2016-07-15

How to Cite

Nosenko, O. M., Dubinina, V. G., & Lysenko, M. A. (2016). Composition of lectin receptors in serous and mucinous ovarian cystadenomas. REPRODUCTIVE ENDOCRINOLOGY, (29), 16–18. https://doi.org/10.18370/2309-4117.2016.29.16-18

Issue

No. 29 (2016)

Section

Scientific Studies

License

Copyright (c) 2016 O. M. Nosenko, V. G. Dubinina, M. A. Lysenko

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Authors who publish with this journal agree to the following terms:

    1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.

    1. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.