Melatonin exchange in infertile women with repeated implantation failures




infertility, repeated implantation failure, endometrium, melatonin, 6-sulfatoxymelatonin


Background. Repeated implantation failure occurs in approximately 10–15% of women undergoing in vitro fertilization and embryo transfer (IVF-ET) cycles. One of the important factors is the changed morpho-functional state of the endometrium and chronodestruction in the woman’s body.

Objective of the study: to determine the characteristics of melatonin (MT) metabolism in infertile women with repeated implantation failures in IVF-ET cycles.

Material and methods. 103 women of reproductive age with infertility and repeated implantation failures who were treated in IVF-ET cycles were observed. Depending on the onset of pregnancy in the current IVF-ET cycle, women were divided into 2 groups: group A – 35 women with the onset of pregnancy, group B – 68 patients without the onset of pregnancy. The control group (group C) included 32 healthy fertile women. The MT level in blood serum and 6-sulfatoxymelatonin (aMT6s) in urine was determined.

Results. The initial levels of MT in patients of group A (11.38 ± 0.38 pg/ml) and in group B (9.55 ± 0.27 pg/ml) were lower than in group C (13.67 ± 0.65 pg/ml) in 1.20 (p < 0.01) and 1.43 (p < 0.01) times, respectively. At the same time, the level of MT in women of group A was 1.19 times higher than in the group B (p < 0.03). Excretion of aMT6s in urine in group A (5.09 ± 0.19 ng/ml Cr) and in group B (3.03 ± 0.09 ng/ml Cr) were lower than in group C (6.12 ± 0.21 ng/ml Cr) respectively in 1.20 (p < 0.01) and 2.02 (p < 0.01) times. The aMT6s concentration in urine in patients of group A was 1.68 times higher than in group B (p < 0.01). A direct relationship was established between the levels of serum MT and urinary aMT6s, which was most adequately approximated by a polynomial dependence: y  = 0.0139x2 – 0.1127x + 3.2978, R² = 0.2994, r = 0.51, p < 0.01.

Conclusions. Women with repeated implantation failures are characterized by a decrease in the level of MT in blood serum and its main metabolite aMT6s in urine, which may be one of the key factors of implantation failure.

Author Biographies

O.M. Nosenko, Odesa National Medical University, Odesa

MD, professor at the Obstetrics and Gynecology Department

R.Y. Demydchyk, Odesa National Medical University, Odesa, Ukraine

PhD student, Department of Obstetrics and Gynecology


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How to Cite

Nosenko, O., & Demydchyk, R. (2024). Melatonin exchange in infertile women with repeated implantation failures. REPRODUCTIVE ENDOCRINOLOGY, (71), 94–102.



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