Collagen metabolism deteriorations in intrauterine septum tissue of women with recurrent pregnancy losses

В. М. Запорожан, В. И. Гладчук, В. Г. Маричереда, Н. Н. Рожковская

Abstract


Formation of an intrauterine septum caused by abnormal embryonic development may be due to changes of the connective tissue, which status causes tissue morphogenesis. Therefore, the aim of this work was to determine the characteristics of collagen in the tissues, which were obtained by resection of intrauterine septum. A further object of the research was to study the characteristics of the collagen synthesis when the therapeutic complex including methylprednisolone, pentoxyphylline and nicotinamide is used at preoperative preparation.

10 patients in the absence of contraindications for 2 weeks before surgery received methylprednisolone, pentoxyphylline and nicotinamide. 10 patients did not receive this medical complex. As a control, was used endometrial tissue of 10 women, resulting in diagnostic hysteroscopy with retrospective normal diagnosis.

When duration of incubation of tissue samples, which was got from traditionally treated women in the course of intrauterine septum resection, was 120 minutes, the level of [3H]-proline inclusion increased by 2.2 times in comparison with the control. Similar incubation of the samples, which were derived from patients who were given complex treatment in preoperative period – methylprednisolone (2.0 mg/day), pentoxyphylline (100 mg/thrice per day) and nicotinamide (10 mg twice per day) was resulted in the reduction of [3H]-proline inclusion (by 37.1%) compared with the analogous index in traditionally treated patients. Nevertheless, this index continued to exceed such one in control group by 60.0%. Besides, proposed treatment induced the increase of the amount of newly synthesized collagen by 25.0% in comparison with traditionally treated patients.

Thus, the results indicate a pathogenetic role of collagen metabolism disorders in the intrauterine septum tissue during habitual miscarriage. Preoperative preparation including the use of methylprednisolone, pentoxyphylline and nicotinamide, reduces the synthesis of collagen in the tissue of endometrial septum.


Keywords


full uterus septum; recurrent pregnancy losses; proline; collagen

References


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GOST Style Citations


1. Серов, В.В., Шехтер, А.Б. Соединительная ткань: функциональная морфология и общая патология. – М.: Медицина. – 1981. – 312 с.

2. Mencaglia, L., Carri, G., Prasciolu, C., et al. “Feasibility and complications in bipolar resectoscopy: Preliminary experience.” Minimally Invasive Therapy & Allied Technologies 22.1 (2013): 50–5.

3. Jowell, P.S., Epstein, S., Fallon, M.D. “1,25-dihudroxyvitamin D3 modulates glucocorticoid-induced alteration in serum bone GLA protein and bone histomorphometry.” Endocrinology 120.2 (1987): 531–6.

4. Keck, K. “An ultramicro techique for the determination of deoxypentose nucleic acid.” Arch Biochem Biophys 63 (1976): 446–51.

5. Olson, G.A., Olson, R.D., Kastin, A.J. “Endogenous opiates: 1993 (Review).” Peptides 15.8 (1994): 1513–56.

6. Noel, P., Nelson, S., Bokulic, R., et al. “Pentoxyfillin inhibits lipopolysaccharide-induced serum tumor necrosis factor and mortality.” Life Sci 47 (1990): 1023–29.

7. Russell, J.E., Walker, W.V., Fenster, R.J. “In vitro evaluation of circadian patterns of bone collagen formation.” Proceed of Society for Exp Biol and Medc 180 (1985): 375–81.

8. Anderson, G.D., Peterson, T.C., Farin, F.M., et al. “The effect of nicotinamide on gene expression in a traumatic brain injury model.” Front Neurosci 2 (2013). DOI: 10.3389/fnins.2013.00021

9. Carranza-Mamane, B., Havelock, J., Hemmings, R., et al. “The management of uterine fibroids in women with otherwise unexplained infertility.” J Obstet Gynecol Can 37.3 (2015): 277–88.

10. Webster, D.F., Harvey, W. “Quantitative assay for collagen synthesis in microwell fibroblast cultures.” Anal Biochem 96 (1979): 220–4.





DOI: http://dx.doi.org/10.18370/2309-4117.2016.30.32-35

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ISSN 2411-1295 (Online), ISSN 2309-4117 (Print)