Collagen metabolism deteriorations in intrauterine septum tissue of women with recurrent pregnancy losses




full uterus septum, recurrent pregnancy losses, proline, collagen


Formation of an intrauterine septum caused by abnormal embryonic development may be due to changes of the connective tissue, which status causes tissue morphogenesis. Therefore, the aim of this work was to determine the characteristics of collagen in the tissues, which were obtained by resection of intrauterine septum. A further object of the research was to study the characteristics of the collagen synthesis when the therapeutic complex including methylprednisolone, pentoxyphylline and nicotinamide is used at preoperative preparation.

10 patients in the absence of contraindications for 2 weeks before surgery received methylprednisolone, pentoxyphylline and nicotinamide. 10 patients did not receive this medical complex. As a control, was used endometrial tissue of 10 women, resulting in diagnostic hysteroscopy with retrospective normal diagnosis.

When duration of incubation of tissue samples, which was got from traditionally treated women in the course of intrauterine septum resection, was 120 minutes, the level of [3H]-proline inclusion increased by 2.2 times in comparison with the control. Similar incubation of the samples, which were derived from patients who were given complex treatment in preoperative period – methylprednisolone (2.0 mg/day), pentoxyphylline (100 mg/thrice per day) and nicotinamide (10 mg twice per day) was resulted in the reduction of [3H]-proline inclusion (by 37.1%) compared with the analogous index in traditionally treated patients. Nevertheless, this index continued to exceed such one in control group by 60.0%. Besides, proposed treatment induced the increase of the amount of newly synthesized collagen by 25.0% in comparison with traditionally treated patients.

Thus, the results indicate a pathogenetic role of collagen metabolism disorders in the intrauterine septum tissue during habitual miscarriage. Preoperative preparation including the use of methylprednisolone, pentoxyphylline and nicotinamide, reduces the synthesis of collagen in the tissue of endometrial septum.

Author Biographies

В. М. Запорожан, Odesa National Medical University

MD, Academician of the NAMS of Ukraine, Rector, professor at the Obstetrics and Gynaecology Department № 1

В. И. Гладчук, Odesa National Medical University

PhD, postgraduate student at the Obstetrics and Gynaecology Department № 1

В. Г. Маричереда, Odesa National Medical University

MD, Prorector, professor at the Obstetrics and Gynaecology Department № 1

Н. Н. Рожковская, Odesa National Medical University

MD, professor at the Obstetrics and Gynaecology Department № 1


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How to Cite

Запорожан, В. М., Гладчук, В. И., Маричереда, В. Г., & Рожковская, Н. Н. (2016). Collagen metabolism deteriorations in intrauterine septum tissue of women with recurrent pregnancy losses. REPRODUCTIVE ENDOCRINOLOGY, (30), 32–35.



Pregnancy and childbirth