Chromosomal abnormalities of embryos as a major factor recurrent spontaneous abortion after artificial and natural fertilization




chromosomal abnormalities, embryo, spontaneous abortion, in vitro fertilization, natural fertilization


Chromosomal abnormalities are the most common causes of abortion in the first trimester. Most miscarriages in this term of pregnancy are due to aneuploidy, the frequency of which is 50–80%. Autosomal trisomy is the most common chromosomal abnormalities.

The aim of the study was to investigate chromosomal abnormalities of abortive material in women with recurrent spontaneous abortion after in vitro fertilization and after natural fertilization.

Cytogenetic and molecular cytogenetic studies include 440 samples of chorionic villi from women with recurrent miscarriage, among whom 240 patient had the pregnancy in IVF cycles (group I) and 200 women had a naturally way pregnancy (group II). The average age of patients was 33 years.

Spectral karyotyping by fluorescent hybridization in situ on interphase nuclei of chorionic villi cells was performed with direct hybridization labeled with fluorescent dyes DNA probes.

Results of cytogenetic and molecular cytogenetic studies showed that 43.33% abortions after IVF and 65.00% ones following natural conception had an abnormal karyotype, including autosomal trisomies respectively 35.00% and 42.00%; disomy X – 1.25% and 0.50%; monosomy X – 2.08% and 3.0%; monosomy 21 – 0.83% and 0.50%; polyploidy – 2.92% and 13.00%; structural chromosomal abnormalities – 0.83% and 4.0%; marker chromosome – 0.42% and 1.00%.

Cytogenetic study of chorionic villi in recurrent spontaneous abortion is an integral part of the diagnostic evaluation. If at the cytogenetic study a spontaneous chromosomal anomaly detected, the next pregnancy proposed planning without a more detailed genetic examination. If a hereditary chromosomal anomaly, it is assessed the risk of its occurrence during the next pregnancy, carried out medical and genetic counseling.

An artificial insemination with controlled selection of sperm and embryos in women with recurrent miscarriage history leads to a smaller number of chromosomal abnormalities in the embryo than in miscarriages after natural conception.

Author Biography

K. P. Holovatiuk, Center of Reproductive Health Protection “Gamete”, Odesa

PhD, chief medical officer of Medical


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How to Cite

Holovatiuk, K. P. (2016). Chromosomal abnormalities of embryos as a major factor recurrent spontaneous abortion after artificial and natural fertilization. REPRODUCTIVE ENDOCRINOLOGY, (27), 48–51.



Pregnancy and childbirth