Immuno-morphological features of endometrial hyperplasic processes
Keywords:endometrial hyperplasia, dendritic cells, apoptosis
The aim of the study was to evaluate the immuno-morphological features of endometrial hyperplastic processes by determining the expression of dendritic cells (CD56+, CD83+, CD1a+) and apoptotic factors (Bcl-2 and Вах) in simple and atypical endometrial hyperplasia.
Materials and methods. The study involved 25 women with simple hyperplasia (group I), 25 – atypical hyperplasia (group II), control group consisted of 20 healthy women. Determination of dendritic cells in the endometrial biopsies performed by immunohistochemical method using monoclonal antibodies LIR, Dardilly (France) to CD56+, CD83+, CD1a+. Expression of the antiapoptosis protein Bcl-2 and proapoptosis protein Вах was observed in the cytoplasm of cells and differed in intensity staining.
Results. Immunohistochemical study of endometrial biopsies found that dendritic cells that expressed antigens CD56+, CD83+, CD1a+, showed a high level of expression. In this case, the severity and nature of immune disorders that accompany pathological endometrial transformation depends not only on the severity of its morphological changes, but also on the duration of the procedure (r = 0,61) and the presence of comorbidity (rA = 0,66). At the same time we found that the degree of expression of factors Bcl-2 and Вах was weak to moderate in women with simple and atypical hyperplasia of the endometrium and negative – in healthy women. The level of expression of markers of apoptosis in group II patients correlated (r = 0,54) with the severity of expression of immature dendritic cells CD1a+. This demonstrates the important role of the functional state of dendritic cells in the regulation of cell proliferation and apoptosis activity in women with endometrial hyperplasia.
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