Features of immunohistochemical markers of stem CELLS CD-117, CD-44 in female placentas on the background of chronic stress





placenta, stem cells, markers C-kit CD-117, CD-44, chronic stress


Background. Placenta is a subject of interest to a wide range of scientists because it is rich in stem cells and their precursors. A stem cell is a cell that has the ability to self-repair and can differentiate into offspring (daughter cells) of one or more germ layers. In recent years, scientists have obtained new data of stem cells regenerative potential. However, only isolated publications about placental stem cells are available. Therefore, our studies about placental stem cells are important for discovery of structural and molecular mechanisms, their changes under the influence of chronic stress.
Objective: to study the features of immunohistochemical markers of pluripotent stem cells and their morphological features.
Materials and methods. We examined 80 women placentas with chronic stress in comparison with control using general histological and immunohistochemical methods in the following groups: group 1 – women placentas with physiological course of pregnancy in term 38–40 weeks, group 2 – women placentas with miscarriage, group 3 – women placentas with chronic stress due to internal irradiation (4.5 Bq/kg and more), group 4 – women placentas which had COVID-19 during pregnancy.
Results. There was a significant increase of stem cell markers expression in the three study groups with a significant predominance in groups 3 and 4. It was also determined the different direction of their active factors.
Conclusions. The general changes of all structures of the placental barrier are detected as a result of chronic stress due to various factors: micro detachment of the decidual membrane (significant increase in cases in the studied groups); malperfusion in the structures of the maternal placental barrier; in the placenta stem cells of the three study groups in comparison with the control were found stress markers. Thus, chronic stress due to various factors causes the same type of changes in placental structures, but they have different degrees of expression – with internal irradiation doses ≥ 4.8 Bq/kg, these changes are most expressive.

Author Biography

Y.M. Bondarenko, SI “O.M. Lukyanova Institute of Pediatrics, Obstetrics and Gynecology of the NAMS of Ukraine”, Kyiv

Junior researcher, Laboratory of Pathomorphology


  1. Mitalipov, S., Wolf, D. “Totipotency, pluripotency and nuclear reprogramming.” Adv Biochem Eng Biotechnol 114 (2009): 185–99.
  2. Koutmani, Y., Karalis, K.P. “Neural stem cells respond to stress hormones: distinguishing beneficial from detrimental stress.” Front Physiol 6 (2015): 77. DOI: 10.3389/fphys.2015.00077
  3. Baergen, R.N. Manual of Pathology of the Human Placenta: Second Edition (2011).
  4. Antypkin, Y.H., Zadorozhna, T.D., Parnytska, O.I. Placental pathology (modern aspects). Kyiv (2016): 127 р.
  5. Khong, T. Yee, et al. Pathology of the Placenta: a Practical Guide (2019).
  6. Pathak, S., Hook, E., Hackett, G., et al. “Cord coiling, umbilical cord insertion and placental shape in an unselected cohort delivering at term: relationship with common obstetric outcomes.” Placenta 31.11 (2010): 963–68. DOI: 10.1016/j.placenta.2010.08.004
  7. Stanek, J. “Membrane microscopic chorionic pseudocysts are associated with increased amount of placental extravillous trophoblasts.” Pathology 42 (2010): 125–30.
  8. Bendon, R.W., Coventry, S.C., Reed, R.C. “Reassessing the clinical significance of chorionic membrane microcysts and linear necrosis.” Pediatr Dev Pathol 15.3 (2012): 213–6. DOI: 10.2350/11-08-1072-OA.1
  9. Reese, J.A., Peck, J.D., Yu, Z., et al. “Platelet sequestration and consumption in the placental intervillous space contribute to lower platelet counts during pregnancy.” Am J Hematol 94.1 (2019): E8–E11. DOI: 10.1002/ajh.25321
  10. Haram, K., Mortensen, J.H., Myking, O., et al. “Early development of the human placenta and pregnancy complications.” J Matern Fetal Neonatal Med 33.20 (2020): 3538–45. DOI: 10.1080/14767058.2019.1578745
  11. McCracken, M.N., George, B.M., Kao, K.S., et al. “Normal and Neoplastic Stem Cells.” Cold Spring Harb Symp Quant Biol 81 (2016): 1–9. DOI: 10.1101/sqb.2016.81.030965
  12. Hara, A., Kato, K., Ishihara, T., et al. “Meflin defines mesenchymal stem cells and/or their early progenitors with multilineage differentiation capacity.” Genes Cells 26.7 (2021): 495–512. DOI: 10.1111/gtc.12855
  13. Witkowska-Zimny, M., Wrobel, E. “Perinatal sources of mesenchymal stem cells: Wharton’s jelly, amnion and chorion.” Cell Mol Biol Lett 16 (2011): 493–514.
  14. Summers, M., Helm, K., Majka, S.M. “Enrichment and Characterization of Human and Murine Pulmonary Mesenchymal Progenitor Cells (MPC ).” Methods Mol Biol 2155 (2020): 125–40. DOI: 10.1007/978-1-0716-0655-1_11
  15. Nishimura, M., Nguyen, L., Watanabe, N., et al. “Development and characterization of novel clinical grade neonatal porcine bone marrow-derived mesenchymal stem cells.” Xenotransplantation 26.3 (2019): e12501. DOI: 10.1111/xen.12501
  16. Kay, H.H., Nelson, D.M., Wang, Y. The Placenta: from Development to Disease. Wiley-Blackwell, West Sussex, United Kingdom (2011): 346 p.
  17. Atala, A. Amniotic Fluid and Placenta Stem Cells. In: Bhattacharya, N., Stubblefield, P. (eds) Regenerative Medicine Using Pregnancy-Specific Biological Substances. Springer, London (2011).
  18. Heazlewood, C., et al. Exploring the Human Term Placenta as a Novel Source for Stem Cells and Their Application in the Clinic (2019).
  19. Yang, H., Wang, F., Liu, X., et al. “Mesenchymal stem cells from human umbilical cord regulate the expression of major histocompatibility complex in human neural stem cells and their lineages.” Neurosci Lett 738 (2020): 135359. DOI: 10.1016/j.neulet.2020.135359
  20. Wang, M., Yang, Y., Yang, D., et al. “The immunomodulatory activity of human umbilical cord blood-derived mesenchymal stem cells in vitro.” Immunology 126 (2009): 220–32.
  21. Chaikovskyi, Y.B., Deltsova, O.I., Herashchenko, S.B. Stem cells: a monograph. Ivano-Frankivsk: City of NV (2014): 500 p.
  22. Angiero, F., Sozzi, D., Seramondi, R., Valente, M.G. “Epithelial-myoepithelial carcinoma of the minor salivary glands: immunohistochemical and morphological features.” Anticancer Res 29.11 (2009): 4703–9.
  23. Zhao, X., Malhotra, G.K., Band, H., Band, V. “Derivation of myoepithelial progenitor cells from bipotent mammary stem/progenitor cells.” PLoS One 7.4 (2012): e35338. DOI: 10.1371/journal.pone.0035338
  24. Kretzschmar, K., Weber, C., Driskell, R.R., et al. “Compartmentalized Epidermal Activation of β-Catenin Differentially Affects Lineage Reprogramming and Underlies Tumor Heterogeneity.” Cell Rep 14.2 (2016): 269–81. DOI: 10.1016/j.celrep.2015.12.041
  25. Schmitt, F., Ricardo, S., Viera, A.F., et al. “Cancer stem cell markers in breast neoplasias: their relevance and distribution in distinct molecular subtypes.” Virchows Arch 460.6 (2012): 545–53.
  26. Shackleton, M. “Normal stem cells and cancer stem cells: similar and different.” Semin Cancer Biol 20.2 (2010): 85–92. DOI: 10.1016/j.semcancer.2010.04.002
  27. Horii, M., et al. “Identification of Subtype-Specific Markers for Preeclampsia Using Placental Pathology and RNAseq.” Reproductive Sciences (Thousand Oaks, Calif) 28 (2021): 168A.
  28. Mangialardi, K., Fanelli, M., Cazzato, G., et al. “Laminar Necrosis and Hypoxic Damage of the Placenta: A Case-Control Study.” Int J Environ Res Public Health 19.7 (2022): 3891. DOI: 10.3390/ijerph19073891
  29. Zadorozhna, T.D., Bondarenko, Y.M. Scientific work “Morphological criteria of syncytiotrophoblast of multinucleated epithelium of placental villi in women with chronic stress”. Сertificate of copyright registration for the work № 112811 (2022).
  30. Khong, T.Y., Mooney, E.E., Ariel, I., et al. “Sampling and definitions of placental lesions: Amsterdam Placental Workshop Group consensus statement.” Arch Pathol Lab Med 140.7 (2016): 698–713.



How to Cite

Bondarenko, Y. (2022). Features of immunohistochemical markers of stem CELLS CD-117, CD-44 in female placentas on the background of chronic stress. REPRODUCTIVE ENDOCRINOLOGY, (65), 71–79. https://doi.org/10.18370/2309-4117.2022.65.71-79



Pregnancy and childbirth