Optimization of diagnostic studies in patients with adenomiosis and/or hyperplastic processes of endometrium





adenomyosis, endometriosis, immunohistochemical study, Ki-67, shear wave elastography


Study objective: to optimize diagnostic measures in patients with adenomyosis and/or hyperplastic endometrial processes (HEP).
Material and methods. The study included 128 patients aged from 27 to 53 years. The first group included 38 (29.6%) patients with a grade I–II adenomyosis, the second group included 41 (32.1%) patients with a combination of grade I–II adenomyosis and HEP and/or uterine leiomyoma, the third group included 39 (30.5%) women with only HEP, the fourth (control) group included 10 (7,8%) women without endometrial pathology. Patients underwent hysteroscopy with targeted biopsy, elucidated the pathomorphological features of connective tissue component of the endometrium and uterus transitional zone, studied specific markers of adenomyosis progression, namely vascular endothelial growth factor and Ki-67 proliferation index, and also determined the parameters of shear wave ultrasound elastography.
Results. Routine histological examination of adenomyosis was confirmed only in 11 (30.6%) cases out of 36 suspected, while HEP (polyps, glandular hyperplasia) and hyperplastic pathology of endocervix (polyps) – in 34 (97.1%) cases out of 35 suspected. Ki-67 antigen expression was positive in the nuclei of epithelial cells in the adenomyosis glands and in the cytogenic stroma cells. A greater value of expression was in the epithelium of glands in superficially located heterotopies – with grade I–II adenomyosis compared with other studied groups. Shear wave elastography showed significant differences in the myometrium color between the first, third and control groups. The unchanged myometrium in all patients of the control group was characterized by staining in shades of blue, while in 9 (90%) patients the color was uniform.
Conclusion. It is recommended to use a shear wave ultrasound elastography in laboratory and instrumental examination with determination of Ki-67 proliferation protein expression and intensification of the neovascularization process in endometrial biopsy specimens to improve the diagnosis of adenomyosis.

Author Biographies

T.V. Mielikhova, Kharkiv National Medical University

Assistant, Obstetrics and Gynecology Department No. 2

O.V. Gryshchenko, Kharkiv Medical Academy of Postgraduate Education

MD, professor, head of the Perinatology, Obstetrics and Gynecology Department

V.V. Lazurenko, Kharkiv National Medical University

MD, professor, head of Obstetrics and Gynecology department No. 2

R.A. Safonov, Kharkiv National Medical University; Kharkiv Regional Clinical Hospital

PhD, associate professor, Obstetrics and Gynecology Department No. 2;

head of the Gynecological Department

O.S. Alekseeva, Kharkiv National Medical University

Graduate student, Obstetrics and Gynecology Department No. 2


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How to Cite

Mielikhova, T., Gryshchenko, O., Lazurenko, V., Safonov, R., & Alekseeva, O. (2021). Optimization of diagnostic studies in patients with adenomiosis and/or hyperplastic processes of endometrium. REPRODUCTIVE ENDOCRINOLOGY, (58), 34–39. https://doi.org/10.18370/2309-4117.2021.58.34-39