Effect of Distreptaza® Distrept on the antibiofilm activity of clindamycin against Staphylococcus spp.





biofilms, Staphylococcus spp., Distreptaza® Distrept, antibacterial agents


This article highlights one of the most important medical and social problems – an increase in the number of diseases of the female reproductive system caused by microorganisms and the insufficient effectiveness of modern antimicrobial medicines, absence of medicine with antibiofilm action. This prompts the authors to search for products that increase the effectiveness of antibiotic therapy, as well as medicines that can suppress film formation and disrupt the biofilm structure. The article presents the results of studying the biofilms sensitivity formed by Staphylococcus spp. to the action of the enzyme preparation Distreptaza® Distrept and its effect on the specific antibacterial activity of clindamycin.

Purpose of the study: to evaluate the Distreptaza® Distrept impact on the specific effect of clindamycin against the biofilms of Staphylococcus aureus and Staphylococcus epidermidis.

Materials and methods: one-day cultures of S. aureus 222 and S. epidermidis 297 were used in the experiments. Solutions of drugs and microorganisms were added simultaneously, with the action on the formed biofilms – 24 hours after the introduction of the bacterial inoculum to study the effect of medicines on film formation. Experiments were carried out according to the generally accepted technique based on the sorption of gentian violet by biofilm structures with subsequent desorption of the dye into an organic solvent.

Results: the experiments showed that Distreptaza® Distrept interferes with the film formation of Staphylococcus spp. (S. aureus inhibition in 85.4%, S. epidermidis in 20.3%). Distreptaza® Distrept affects the biofilms formed by S. aureus, the biomass of S. aureus decreases by 59.5%. It was found that Distreptaza® Distrept enhances the specific activity of clindamycin, both at the stage of film formation, and on the formed biofilms.

Conclusions: the medicine Distreptaza® Distrept has antibiofilm activity, increases the specific antibacterial effect of clindomycin against biofilms of gram-positive microorganisms. This allows us to talk about the prospects for this drug use as monotherapy in chronic recurrent diseases of the genital tract, as well as in combination with antimicrobial drugs.

Author Biographies

N. O. Vrynchanu, SI “Institute of Pharmacology and Toxicology of the NAMS of Ukraine”

MD, head of the Laboratory of Pharmacology of Antimicrobial Agents

N. I. Hrynchuk, SI “Institute of Pharmacology and Toxicology of the NAMS of Ukraine”

Junior researcher

V. V. Samsonova, SI “Institute of Pharmacology and Toxicology of the NAMS of Ukraine”

General practitioner


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Inflammatory diseases