Peculiarities of the inflammatory reactions development in placenta at delivery in different gestational terms
Keywords:placenta, immunohistochemical analysis, CD45 expression in placenta, TNF-α expression in placenta, childbirth.
Objective of the study: to study peculiarities of the inflammatory reactions development in placenta according to results of a general histological and immunohistochemical study of the prevalence and intensity of reactions caused by CD45 and tumor necrosis factor α (TNF-α) during childbirth at different gestational periods.
Materials and methods. Morphological and immunohistochemical studies of various placental structures and CD45 and TNF-α expression level in 142 women with preterm delivery at different gestational periods were performed: group I – at 23–29 weeks, group II – at 30–34 weeks; group III – at 35–36 weeks + 6 days, group IV (control) – 40 women at 38–40 weeks.
Results. Inflammatory infiltration and the most expressive CD45 expression prevailed in the decidual membrane, TNF-α expression was predominantly negative in group I. In group II, inflammatory changes were evidenced by collagenization and stromal fibrosis, a decrease in fetal vessels, fibroblast proliferation, an increase in intervillous fibrinoid, and CD45 spread was expressed in decreasing order of intensity in decidual membrane, in stroma of intermediate villi and in stem villi. In group III, foci of inflammatory infiltration spread from the decidual membrane to the intervillous space and villi stroma, vessels plethora of all types of villi, dystrophic changes, and fibrinoid volume increased. CD45 expression was more common and expressive than in the groups I and II, and TNF-α expression was weakly positive only in the decidual membrane. The control group determined the widespread expressive CD45 expression in all placental structures and positive moderate TNF-α expression.
Conclusions. Preterm delivery and delivery on term occur when there is an activation of inflammatory reactions in placenta. Inflammation in the placental structures is a necessary condition for development of the mother-fetal interphase, however, the features of local immune response prove the need to determine genetic mechanisms of the preterm labor.
- Antypkin, Y.G., Zadorozhnaya, T.D., Parnitskaya, O.I. Pathology of the placenta (modern aspects). Kyiv. Atopol LLC (2016): 127 p.
- Batrak, N.V., Malyshkina, A.I., Kroshkina, N.V. “Immunological aspects of habitual miscarriage.” Obstetrics and gynecology 12 (2014): 10–4.
- Vovk, I.B., Kondratyuk, V.K., Trokhimovich, O.V., Kovalenko, A.I. “Threat of early termination of pregnancy: modern views on the etiology, pathogenesis, diagnosis and treatment.” Women's health 5.71 (2012): 50–6.
- Vorobyova, I.I., Zhyvetskaya-Denisova, A.A., Rudakova, N.V., et al. “Features of local immunity in women with miscarriage.” Perinatology and pediatrics 4.72 (2017): 49–53.
- Vorobyova, I.I., Zhyvetskaya-Denisova, A.A., Tkachenko, V.B., et al. “Miscarriage: modern views on the problem.” Women's health 3.119 (2017): 113–7.
- Vorobyova, I.I., Skripchenko, N.Y., Livshits, L.A., et al. Immuno-genetic approaches to the diagnosis of miscarriage as a multifactorial disease (Guidelines). Kyiv (2016): 30 p.
- Vorobyova, I.I., Skripchenko, N.Y., Tkachenko, V.B., Zhyvetskaya-Denisova, A.A. “The role of molecular-genetic and psychosocial aspects of the development of miscarriage in women of the Ukrainian nation.” A word about health 3.15 (2018): 14.
- Sementsova, N.A., Ponomareva, Y.N., Barsanova, T.G., Zairatyants, O.V. “Violation of the processes of angiogenesis, apoptosis and proliferation in the tissues of the ovum and endometrium during an undeveloped pregnancy in the first trimester.” Clinical and experimental morphology 3 (2012): 11–6.
- Sidelnikova, V.M., Sukhikh, G.T. Miscarriage: A Guide for Practitioners. Moscow. Medical Information Agency LLC (2010): 536 p.
- Khachatryan, N.A., Krechetova, L.V., Tetruashvili, N.K. “Alloimmune mechanisms of habitual miscarriage.” Obstetrics and gynecology 5 (2014): 3–8.
- Yurova, K.A., Khaziakhmatova, O.G., Sokhonevich, N.A., et al. “Alternative splicing of the CD45 molecule in the mechanisms of molecular genetic control of T-cell differentiation.” Russian immunological journal 2 (2015): 186–93.
- Alijotas-Reig, J., Llurba, E., Gris, J.M. “Potentiating maternal immune tolerance in pregnancy: a new challenging role for regulatory T cells.” Placenta 35 (2014): 241–8.
- Azizieh, F.Y., Raghupathy, R.G. “Tumor necrosis factor-α and pregnancy complications: a prospective study.” Med Princ Pract 24 (2015): 165–70.
- Binder, N.K., Evans, J., Gardner, D.K. “Endometrial signals improve embryo outcome: functional role of vascular endothelial growth factor isoforms on embryo development and implantation in mice.” Human Reproduction and Embryology 29.10 (2014): 2278–86.
- Matson, B.C., Caron, K.M. “Uterine natural killer cells as modulators of the maternal-fetal vasculature.” Int J Dev Biol 58 (2014): 199–204.
- Chatterjee, P., Chiasson, V.L., Bounds, K.R., Mitchell, B.M. “Regulation of the anti-inflammatory cytokines interleukin-4 and interleukin-10 during pregnancy.” Front Immunol 27.5 (2014): 253.
- Cheng, S.B., Sharma, S. “Interleukin-10: a pleiotropic regulator in pregnancy.” Am J Reoprod Immunol 73 (2015): 487–500.
- Jabrane-Ferrat, N., Siewiera, J. “The up side of decidual natural killer cells: new developmentsin immunology of pregnancy.” J Immunol 141 (2014): 490–549.
- Kajdaniuk, D., Marek, B., Borgiel-Marek, H., Kos-Kudła, B. “Transforming growth factor β1 (TGFβ1) in physiology and pathology.” Endokrynol Pol 64.5 (2013): 384–96.
- Okada, H., Tsuzuki, T., Shindoh, H., et al. “Regulation of decidualization and angiogenesisin the human endometrium.” Journal of Obstetrics and Gynaecology Rasearch 40.5 (2014): 1180–7.
- Shimono, A., Imoto, Y., Sakamoto, H. “An immunohistochemical study of placental syncytiotrophoblasts in neonatal hemochromatosis.” Placenta 48 (2016): 49–55.
- Suzuki, S., Ouchi, N. “T-helper-1 / T-helper2 Cell Immunity in Preeclamptic Twin Pregnancy.” J Nippon Med Sch 6 (2007): 434–6.
- Tilburgs, T., Roelen, D.L., van der Mast, B.J., et al. “Differential distribution of CD4+ CD25bright and CD8+ CD28– T-cells in decidua and maternal blood during human pregnancy.” Placenta 27 Suppl. A (2006): 47–53.
- van Well, G.J., Daalderop, L.A., Wolfs, T., Kramer, B. “Human perinatal immunity in physiological conditions and during infection.” Mol Cell Pediatr 4 (2017): 4.
- Yuan, J., Li, J., Huang, S.Y., Sun, X. “Characterization of the subsets of human NKT-like cells and the expression of Th1/Th2 cytokines in patients with unexplained recurrent spontaneous abortion.” J ReprodImmunol 110 (2015): 81–8.
How to Cite
Copyright (c) 2020 І. І. Воробйова, Н. Я. Скрипченко, В. Б. Ткаченко, С. М. Толкач, С. М. Раздайбєдін
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.