Use of placenta cryoextract for restoring the morphofunctional state of reproductive organs in animals with syndrome of premature ovarian insufficiency. Experimental substantiation
Keywords:experimental model, premature ovarian failure, placenta cryoextract.
Objective of the study was to determine the placenta cryoextract impact on the restoration of the morphofunctional ability of the BALB/c mice uterus and ovaries after modeling their premature ovarian failure syndrome (POF) in the experiment.
Materials and methods. A model of POF was created in 60 BALB/c mice which were divided into 2 groups of 30 animals: group 1 – with a model of POF without treatment, group 2 – with a model of POF and treatment with a placental cryoextract. The 3 (control) group included 30 intact BALB/c mice.
Results. 4 weeks after creation of experimental POF in animals of group 1 histological examination of ovary showed an increase in interstitial tissue, replacing the generative structures of the ovary. An increased number of fibroblast cell elements and collagen fibers were also shown, indicating the growth of the organ stroma. Weight dropped sharply to 17.5 g, followed by a slow recovery in all animals of groups 1 and 2 after reproduction of the POF model. In this case, the weight in animals of group 2corresponded to the indices of control group already at 5th week and amounted to 21 g, reaching 22.1 g at 8th week. Animals in the 1 group restored their weight to 21.1 g only at 8th week. 5 weeks after POF simulation a complete restoration of estrous cycles occurred in 20% of in group 1 and in 70% in group 2. At week 8th the number of effective mating in group 1 was only 30%, while in group 2 this indicator increased to 80%.
Conclusion. Intraperitoneal administration of cryopreserved placenta extract in mice with experimentally simulated POF leads to restoration of uterus morphological structure and partial restoration of ovarian structure with appearance of follicle-like generative elements in them.
- Nishida, M., Miyamoto, Y., Kawano, Y., Takebayashi, K. “A case of successful laparoscopic surgery for tubal stump pregnancy after tubectomy.“ Clin Med Insights Case Rep 8 (2015): 1–4.
- Chirculescu, B., Chirculescu, R., Ionescu, M., et al. “Complete Tubal Abortion: A Rare Form of Ectopic Pregnancy.“ Chirurgia (Bucur) 1.112 (2017): 68–71.
- Karahasanoglu, A., Uzun, I., Ozdemir, M., Yazicioglu, F. “Human Chorionic Gonadotropine in Cul-de-sac Fluid in Tubal Ectopic Pregnacy; A NewDiagnostic Approach.“ J Clin Diagn Res 4.10 (2016): QC01–3.
- The American College of Obstetricians and Gynecologists. “ACOG practice bulletin No. 191: Tubal Ectopic Pregnancy.“ Obstet Gynecol 131.2 (2018): e65-e77. DOI: 10.1097/AOG.0000000000002464
- Khan, Z., Lindheim, S.R. “In pursuit of understanding interstitial pregnancies: a rare yet high-risk ectopic pregnancy.“ Fertil Steril 2.112 (2019): 246–7.
- Cherednichenko, V.I., Trotsenko, P.N., Pikul, M.Y., Mezina, V.I. “Medical treatment of tubal disease.” In: Proceedings of the Association of Obstetricians-Gynecologists of Ukraine. Kyiv (2012): 425–7.
- Aboud, E, Chaliha, C. “Nine year survey of 138 ectopic pregnancies.” Archives of Gynecology & Obstetrics 2.261 (2010): 83–7.
- Bernstein, H.B., Thrall, M.M., Clark, W.B. “Expectant management of intramural ectopic pregnancy.” Obstet Gynecol 5.97 (2010): 826–7.
- Ego, A., Subtil, D., Di Pompeo, C. “Patient satisfaction with management of ectopic pregnancy.“ Eur J Obstet Gynecol Reprod Biol 1.98 (2010): 83–90.
- Hsu, J.Y., Chen, L., Gumer, A.R., Tergas, A.I. “Department Disparities in the Management of Ectopic Pregnancy.“ Am J Obstet Gynecol 1.217 (2017): 49.e1–49.e10.
- Li, H., Liu, Y., Wen, S., et al. “Evaluation of serum biomarkers and efficacy of MTX in women with ectopic pregnancy.“ Mol Med Rep 3.20 (2019): 2902–8.
- Paull, C., Robson, S. “Hospital Admission and Surgical Approach to Tubal Ectopic Pregnancy in Australia 2000 to 2014: A Population-Based Study.“ Aust N Z J Obstet Gynaecol 2.58 (2018): 234–8.
- Song, T., Lee, D.H., Kim, H.C., Seong, S.J. “Laparoscopic Tube-Preserving Surgical Procedures for Ectopic Tubal Pregnancy.“ Obstet Gynecol Sci 6.59 (2016): 512–8.
- “Tubal infertility: a practical look at the problem.” Female doctor 2.82 (2017): 60–5.
- Sholapurkar, S.L. “Diagnostic and management modalities in early tubal ectopic pregnancy with focus on safety.” Hum Reprod Update 5.21 (2015): 692–3.
- Atilgan, R., Kuloglu, T., Bostosun, A., Orak U. “Investigation of the effects оf unilateral total salpingectomy on ovarian proliferating cell nuclear antigen and follicular reserve: experimental study.“ Eur J Obstet Gynecol Reprod Biol 1.188 (2015): 56–60.
- Rashid, M., Osman, S.H., Khashoggi, T.Y. “Factors affecting fertility following radical versus conservative surgical treatment for tubal pregnancy.“ Saudi Med J 4.22 (2010): 337–41.
- Olug, P., Oner, G. “Evaluation of the effects or single or multiple dose methotrexate administration, salpingectomy on ovarian reserve of rat with the measurement of anti-Mullerian hormone (AMH) levels and histological analysis.” Eur J Obstet Gynecol Reprod Biol 181 (2014): 205–9.
- Park, E.H.G., Mohammadi-Zaniani, G., Pronin, S., Elderfield, C.H.J. “Subsequent pregnancy outcome of tubal ectopic pregnancies treated by methotrexate and salpingectomy.” Eur J Obstet Gynecol Reprod Biol 212 (2017): 192–3.
- Hu, C., Chen, Y., Hou, H., et al. “Analysis of reoperation for infertility women with tubal pregnancy after conservative surgery.” Zhonghua Yi Xue Za Zhi 11.94 (2014): 848–51.
- Cheng, X., Tian, X., Yan, Z., et al. “Comparison of the Fertility Outcome of Salpingotomy and Salpingectomy in Women with Tubal Pregnancy: A Systematic Review and Meta-Analysis.” PLoS One 3.11 (2016): e0152343.
- Prokopiuk, O.S. “Placer cryopreservation and determination of mechanisms of its influence on the organism of the recipients of late ontogeny.” Thesis abstract for MD degree. Kharkiv (2014): 36 p.
- Xiao, G.Y., Liu, I.H., Cheng, C.C., Chang, C.C. “Amniotic fluid stem cells prevent follicle atresia and rescue fertility of mice with premature ovarian failure induced by chemotherapy.” PLoS One 9.9 (2014): e106538.
- Grishchenko, V.I., Yurchenko, T.N., eds. Placenta: cryopreservation, structure, properties, prospects for clinical use. Kharkiv: SPD FL Brovin A.V. (2011): 292 p.
How to Cite
Copyright (c) 2020 М. М. Козуб
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.