Morphological changes in the internal organs of mice with a simulated ovarian hyperstimulation syndrome

Э. М. Айзятулова


One of the serious complications of in vitro fertilization, threatening the health and life of patients, is ovarian hyperstimulation syndrome. Since the study of the morphology of the internal organs in a woman with this syndrome is complicated, and sometimes impossible, the creation of an experimental model of this pathology using laboratory animals is relevant.

Objective of the study is to evaluate the morphofunctional changes in the internal organs of mice with simulated ovarian hyperstimulation syndrome, by stimulating the superovulation of animals ovaries by exogenous gonadotropic hormones in high doses.

Materials and methods. The experiment was carried out on females of hybrid mice (СBA×C57BL) weighing 18–20 g. To simulate ovarian hyperstimulation syndrome, 8 animals were intraperitoneally injected with 20 IU of mare serum gonadotropin, after 48 h – 7.5 IU of human chorionic gonadotropin. The control group consisted of 8 animals at the estrus stage, which corresponded to spontaneous ovulation. For the histological study, the fragments of animal organs were fixed in 10% neutral formalin, carried out through alcohols of increasing concentration and embedded in paraffin, histological sections were made, which were clarified in xylene and stained with hematoxylin and eosin.

Results. Unlike intact mice, the parenchyma of the internal organs of which correspond to the morphological norm, persistent disorders of angiopathogenic and histoarchitectonics, microcirculation disorders that lead to hyperemia, ischemia, transudation and accumulation of fluid in tissues and interstitial lumen, inflammation of organs are develop in the inner organs of mice with simulated ovarian hyperstimulation syndrome.

Conclusion. Unlike intact mice the parenchyma of the internal organs of which corresponds to the morphological norm, vital internal organs of mice with modeled ovarian hyperstimulation syndrome are involved in the pathological process. Animal studies indicate the need for preventive measures to reduce the frequency of ovarian hyperstimulation syndrome for women, in particular, prudent prescribing doses of gonadotropic hormones in controlled ovarian stimulation of ovulation, since ovarian hyperstimulation syndrome leads to pathological processes of internal organs, significantly disrupting their function, sometimes irreversibly.


ovarian hyperstimulation syndrome; experimental modeling; mice; gonadotropic hormones; internal organs

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Bhavsar, P.N., Padwal, N.J., Bhide, M., et al. “Life-threatening medical complications due to ovarian hyperstimulation syndrome: a hidden etiology.” J Assoc Physicians India 65.11 (2017): 87–91.

Corbett, S., Shmorgun, D., Claman, P. “The prevention of ovarian hyperstimulation syndrome.” J Obstet Gynaecol Can 36.11 (2014): 1024–33. DOI: 10.1016/S1701-2163(15)30417-5

Niederberger, C., Pellicer, A., Cohen, J., et al. “Forty years of IVF.” Fertil Steril 110.2 (2018): 185–324.e5. DOI: 10.1016/j.fertnstert.2018.06.005

Practice Committee of the American Society for Reproductive Medicine. “Prevention and treatment of moderate and severe ovarian hyperstimulation syndrome: a guideline.” Fertil Steril 106.7 (2016):1634–47. DOI: 10.1016/j.fertnstert.2016.08.048

Smith, V., Osianlis, T., Vollenhoven, B. “Prevention of ovarian hyperstimulation syndrome: a review.” Obstet Gynecol Int (2015): 514159. DOI: 10.1155/2015/514159

Szabó, G., Árokszállási, A., Pócsi, D., et al. “Pathophysiology and current clinical approach of ovarian hyperstimulation syndrome.” Orv Hetil 159.34 (2018): 1390–8. DOI: 10.1556/650.2018.31152

Nelson, S. M. “Prevention and management of ovarian hyperstimulation syndrome.” Thromb Res 151 Suppl 1 (2017): S61–S64. DOI: 10.1016/S0049-3848(17)30070-1

Hambartsoumian, E., Tshzmachyan, R. “Ovarian hyperstimulation syndrome free IVF clinics. Myth or reality.” Int J Gynecol Reprod Sci 1.1 (2018): 1–2.

Calabro, R.S, Gervasi, G., Leo, A., et al. “Neurovascular complications of ovarian hyperstimulation syndrome (OHSS): from pathophysiology to recent treatment options.” Recent Pat Endocr Metab Immune Drug Discov 8.2 (2014): 109–16.

Sarikaya, E., Deveer, R., Kiliç, S., Batioğlu, S. “Ongoing twin pregnancy in an obese, polycystic patient with early critical ovarian hyperstimulation syndrome and severe liver dysfunction: case report.” Turkiye Klinikleri J Med Sci 32.1 (2012): 226–30. DOI: 10.5336/medsci.2009-16399

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