Role of gene polymorphism of IL-4 and IL-17 in recurrent miscarriage, came in art cycles
Keywords:assisted reproductive technology, recurrent miscarriage, typing of cytokine genes, IL-4, IL-4R, IL-17A, IL-17F, gene polymorphism
Most researchers suggest that more than 50% of pregnant women after use of assisted reproductive technologies (ART) are faced with the problem of miscarriage, especially in the first trimester.
The aim of the study was to investigate the frequency of genotypes and allelic variants of the IL-4 (S589T and S33T), IL-4R (Q576R), IL-17A (G197A) and IL-17F (488T/C) genes, depending on the reproductive status and assessment of their association with recurrent miscarriage, which occurred in ART cycles.
Peripheral blood of 240 patients – the eastern Slav with habitual miscarriage, came in ART cycles, and 100 apparently healthy fertile women in the control group with a history of at least one term delivery and lack of spontaneous abortion episodes was studied. The groups were representative of age.
DNA for molecular genetic studies of nuclei isolated from peripheral blood lymphocytes. The method is based on the destruction of lymphocytes using lysis buffer without affecting the integrity of the membranes of lymphocytes nuclei. For typing single nucleotide polymorphisms of IL-4 immune response, IL-4R, IL-17A and IL-17F using polymerase chain reaction product with the melting reaction in the presence of “adjacent” oligonucleotides.
It was found that the genotype CT-33 (OR 3.99; 95% CI 2,42–6,57) and CT-589 (OR 3.99; 95% CI 2,42–6,57) gene IL-4; genotype GG576 (OR 5.95; 95% CI 1,39–25,58) gene IL-4R and TT488 genotype (OR 2.33; 95% CI 1,39–3,91) and CC488 (OR 2.27; 95% CI 0,76–6,84) IL-17F gene were associated with a significant increase in the risk of miscarriage. On the other hand, no significant differences were found between the control group and the basic frequency of polymorphisms IL-17A-G197A (rs227593).
The results of the molecular-genetic typing it possible to identify in the Eastern Slavic population of residents of Odesa region of Ukraine immunogenetic markers of susceptibility/ resistance to the development of recurrent miscarriage. It is shown that cytokine gene typing can be used as a method of early diagnosis and pregravid prognosis of immune forms of reproductive loss in women.
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